THE VITAMIN B12 AND FOLATE PATHOLOGY INVESTIGATION
THE INVESTIGATION OF ERRORS IN PATHOLOGY TESTS
FOR VITAMIN B12 AND FOLATE DEFICIENCY
BY MEANS OF MEDICAL EXPERIMENTS
THE HOMOCYSTEINE INVESTIGATION
THE HCY EXPERIMENT
Summary
Method
Observations
Results - Notes
Results - Series 1
Results - Series 1b
Conclusions
Assay Quality

None of the three labs to measure my homocysteine, SNP, Westmead and PaLMS, gave results that were entirely consistent within all pairs of identical samples. This indicates the possibility of either sample mishandling or antibody interference. The "loss" of all 29 samples by RPAH has prevented any further investigation of these anomalies.

The Westmead homocysteine results were the only ones to indicate the onset of cellular vitamin B12 deficiency, consistent with the Westmead MMA results. These results were also the only ones consistent with the known relationship between serum vitamin B12 and homocysteine levels.

The homocysteine results from SNP and PaLMS were very significantly lower than those from Westmead, never indicating a vitamin B12 deficiency.

The SNP homocysteine results also included an anomaly, which cannot be explained by my biological variation, indicating a serious problem with quality control of the immunoassay.

The errors were, in my opinion, contributed to by failure in quality control. The allowed control ranges, for some immunoassays, are so wide that results obtained using these methods cannot, in my opinion, be relied on for diagnosis or monitoring of vitamin B12 deficiency.

Sullivan Nicolaides Pathology, acknowledges that it relies on the controls and standards supplied by the kit manufacturer, for the measurement of homocysteine, and has no knowledge of the existence of the primary standard for homocysteine, NIST SRM 1955.

Having regard to the results of my investigation, set out in this web site, in my opinion, Sullivan Nicolaides Pathology and PaLMS homocysteine results cannot be relied on for the diagnosis or monitoring of vitamin B12 deficiency.

This web site is owned, designed and maintained by Paul Henry Golding of Nambour, QLD, Australia. © 2007-2016